Perform Probabilistic Quotient Normalization
normalize_data_pqn(x, pqn_reference = "median", pgn_reference_sample = NULL)matrix to normalize. row is variable and column is sample.
normalization reference: "mean" for using the overall average of variables as reference or "median" (default) for using the overall median of variables as reference
vector of index to specify samples which average to use as reference
Normalized expression dataset.
First a total area normalization should be done before PQN is applied.
Dieterle, F., Ross, A., Schlotterbeck, G. & Senn, H. Probabilistic Quotient Normalization as Robust Method to Account for Dilution of Complex Biological Mixtures. Application in H1 NMR Metabonomics. Anal. Chem. 78, 4281-4290 (2006).